I don't have too much to say here but I have always felt there is a half decent transgender read on most of the cast, primarily because of how homestuck handles the topics of gender and in particular, patriachy and toxic masculinity.

When the big bad person in control of the entire story is just a literal stunted man child and the one who ultimately defeats him are his sister and Vriska fucking Serket, the semblence of transgender messaging here is kinda apparent.

I do also think it is worth viewing a lot of the ways homestuck handles gender under the retrospective lens that we KNOW Hussie is nonbinary, much like how the same analytical lens is used for Celeste or perhaps the Matrix. I don't want to be parasocial about this but when Hussie describes most of the cast as "pseudo self inserts" a certain picture is painted.