if i could share my two sents on this situation:

im a trans woman, to me roxy and callie both read incredibly strongly as transfem coded, and i feel like nearly all evidence in the text of the original comic points to that. so to me, the choice with roxy and callies gender feels like transfem erasure (not maliciously mind you), and its personally frustrating. especially when trying to acknowledge transfem roxy or saying that you prefer roxy as a trans woman gets you branded a transphobe, like this thread does.