I could tell you which characters are straight. That one's easy, Jade, Aradia, Meulin, Kurloz, Ms. Paint, Wayward Vagabond, Writ Keeper. Those are all the straight characters in Homestuck.

But cis? Damn. I mean. I'm not saying NONE of them could be cis. Plenty probably are. But saying which ones for sure? Jeez, That's a tall order. Can we say the ones that are objectively evil and terrible, like Cronus, are all definitely cis? No? That's irrational and also gatekeeping? Well, I suppose you're right. Transition isn't something to be permitted or denied based on jumping through enough moral hoops. But can I still kick Cronus off a cliff? Yes you may, says the higher inner voice. I kick Cronus off a cliff. SHE (!!! >:) did i just blow your mind or what?) is dashed to pieces on the rocks below, in a way that is cartoonish, amusing and nonvisceral. Wow, so now you're kicking your fellow women and trans people off a CLIFF, the higher inner voice says? Wow, somebody CANCEL this beeyatch, the higher inner voice continues. Oh my god stoppp!! You said it was fine!! You said I could kick Cronus off a cliff!! You CAN do anything, Magic Mirror. But SHOULD you, the higher inner voice smugs me eyebrowingly? YES!! YOU SHOULD ABSOLUTELY KICK CRONUS OFF A CLIFF!! THAT SEEMS PRETTY BASIC!! SHE HAS COMMITTED FUNDAMENTAL CRIMES!! Well, good point, the higher inner voice teas sippingly.